Yilmaz, E2025-01-272025-01-2720030959-3993https://doi.org/10.1023/A:1023216301165https://hdl.handle.net/20.500.12428/23889Some microbial lipases bio-imprinted with the reversible lipase inhibitor, Orlistat. Comparison of interesterification activities indicated that Orlistat bio-imprinting is equally effective as amphiphile imprinting and yields a 3.5-4.0-fold activity enhancement. Solvent-free incubation medium was as effective as hexane medium. Water addition into the incubation medium erased the bio-imprinting effect; hence, lid opening should be the mechanism of activation. This study provides additional proof to the hypothesis that lipase bio-imprinting is an active-site related phenomenon.eninfo:eu-repo/semantics/closedAccessbio-imprintingenhancementinteresterificationlipaseOrlistatArticle19216116510.1023/A:1023216301165Q4WOS:0001821321000092-s2.0-0038069176Q2