Uygun, Zihni OnurSezgintürk, Mustafa Kemal2025-01-272025-01-2720110003-26701873-4324https://doi.org/10.1016/j.aca.2011.08.044https://hdl.handle.net/20.500.12428/23772In the presented research, a novel, ultra sensitive biosensor for the impedimetric detection of vascular endothelial growth factor (VEGF) is introduced. The human vascular endothelial growth factor receptor 1 (VEGF-R1, Flt-1) was used as a biorecognition element for the first time. The immobilization of VEGF-R1 on glassy carbon electrodes was carried out using layer-by-layer covalent attachment of VEGF-R1. The electrochemical properties of the layers constructed on the electrodes were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The differences in electron transfer resistance (R-er) between the working solution and the biosensor surface, recorded by the redox probe K-3[Fe(CN)(6)]/K-4[Fe(CN)(6)], confirmed the binding of VEGF to VEGF-R1. The new biosensor allowed a detection limit of 100 fg mL(-1) with a linear range of 100-600 fg mL(-1) to be obtained. The biosensor also exhibited good repeatability (with a correlation coefficient of 1.95%), and reproducibility. (C) 2011 Elsevier B.V. All rights reserved.eninfo:eu-repo/semantics/closedAccessTumor growthCancerVascular endothelial growth factorVascular endothelial growth factor receptor-1Electrochemical impedance spectroscopyBiosensorArticle706234334810.1016/j.aca.2011.08.044Q1WOS:0002971846000192-s2.0-8485667394022023871Q1