Büyük, BaşakAydeğer, CemreÖztopuz, ÖzlemOvalı, Mehmet AkifMakav, MustafaEroğlu, Hüseyin Avni2023-09-092023-09-092023Büyük, B., Aydeğer, C., Öztopuz, Ö., Ovalı, M. A., Makav, M., & Eroğlu, H. A. (2023). Effects of Ozone Therapy on Chronic Arsenic Poisoning in Rats. Biological Trace Element Research, 201(8), 3951–3960. https://doi.org/10.1007/s12011-022-03486-y0163-49841559-0720https://doi.org/10.1007/s12011-022-03486-yhttps://hdl.handle.net/20.500.12428/4534Arsenic (As) is a toxic metalloid that affects many organs through drinking water. This study aims to examine the efficacy of ozone therapy on chronic arsenic toxicity. Twenty-four male Wistar albino rats were housed in individual cages and grouped as control, As, O3, and As + O3. As was applied by adding 5 mg/kg/day in drinking water for 60 days. Ozone therapy was applied at 0.5 mg/kg/day (i.p.) O3 in the last 5 days of the experimental period. Tissues were harvested and analyzed for histopathological injury and apoptotic markers. There was no significant difference between the As + O3 and O3 groups (p = 0.186 and p = 0.599) for light microscopic criteria: inflammatory cell infiltration and hydropic degeneration in liver tissue. In TUNEL assessments, similar outcomes were obtained in the control and As + O3 groups. A statistically significant increase was observed in p53 and Caspase 3 (Casp-3) expression levels in the As group compared to the O3 and As + O3 groups. There was no significant difference between the As + O3 and O3 groups on peritubular hemorrhage and desquamation parameters in kidneys (p = 0.147 and p = 0.094). The KIM-1 expression level was significantly increased in the As group compared to the As + O3 group (p = 0.01), and the Casp-3 expression level was not significantly changed in the O3 group compared to the As + O3 group (p = 0.59). In conclusion, it is determined that ozone therapy has ameliorative effects on the microscopic injury of liver and kidney tissues. In addition to microscopic improvement, KIM-1 gene expression levels were ameliorated in the kidneys. The apoptotic cell counts and the Casp-3 and p53 gene expression levels were decreased by O3 administration. Thus, ozone therapy can be a treatment choice for As toxicity.eninfo:eu-repo/semantics/closedAccessArsenic poisoningOzoneExperimentalKIM-1Liver injuryRenal injuryArticle20183951396010.1007/s12011-022-03486-yQ2WOS:0008890500000012-s2.0-8514272958636437433