Karakaya, SerkanDilgin, Yusuf2025-01-272025-01-2720190003-27191532-236Xhttps://doi.org/10.1080/00032719.2018.1509986https://hdl.handle.net/20.500.12428/27662A novel flow-injection amperometric method was proposed for the sensitive and enzymeless determination of hydrogen peroxide based on its electrocatalytic reduction at a palladium nanoparticle-modified pretreated pencil graphite electrode in a laboratory-constructed electrochemical flow cell. Cyclic voltammograms of the unmodified and modified electrodes were recorded in pH 7.0 phosphate buffer containing 0.10 M KCl at a scan rate of 50mV s(-1) for the investigation of electrocatalytic reduction of hydrogen peroxide at the palladium nanoparticle-modified pretreated pencil graphite electrode. Cyclic voltammograms of the pretreated pencil graphite electrode revealed an irreversible oxidation peak and a weak reduction peak of hydrogen peroxide at +1100mV and -450mV vs. an Ag/AgCl/KCl saturated reference electrode. However, the reduction of hydrogen peroxide was observed at -100mV with an increase in current in the cyclic voltammograms of the palladium nanoparticle-modified pretreated pencil graphite electrode compared to the unmodified electrode. These results indicate that the palladium nanoparticle-modified pretreated pencil graphite electrode exhibits efficient electrocatalytic activity for the reduction of hydrogen peroxide. A linear concentration range was obtained between .01 and 10.0mM hydrogen peroxide with a detection limit of 3.0 mu M from flow injection amperometric current-time curves recorded in pH 7.0 phosphate buffer at -100mV and a 2.0mL min(-1) flow rate. The novelty of this work relies on its use of a laboratory-constructed flow cell constructed for the pencil graphite electrode using these inexpensive, disposable, and electrochemically reactive modified electrodes for the amperometric determination of hydrogen peroxide in a flow injection analysis system.eninfo:eu-repo/semantics/closedAccessAmperometryflow injection analysishydrogen peroxidepalladium nanoparticlespencil graphite electrodeArticle526998101710.1080/00032719.2018.1509986Q4WOS:0004616112000102-s2.0-85055553850Q2