Allito, LemiyeComert Onder, FerahDemirel, RamazanOnder, AlperOzden, OzkanErdogan, Musa2026-02-032026-02-0320251756-89191756-8927https://doi.org/10.1080/17568919.2025.2533003https://hdl.handle.net/20.500.12428/34728AimsThe quinolone scaffold is a crucial member of the heterocyclic compound family in modern medicinal chemistry, exhibiting a broad range of biological activities. Since 4-quinolones are known to interact with significant drug targets, and due to the remarkable pharmacological properties of 1,2,3-triazole compounds, a molecular hybridization approach was used to design novel 7-methoxyquinolone-substituted triazole hybrid conjugates (QN1-QN11).Materials and methodsThese hybrid compounds were evaluated to determine their anticancer activities in various breast and colon cancer cell lines, including BT20, MDA-MB-231, MCF7, and HT29. In addition, the apoptotic-like morphological changes in aggressive MDA-MB-231 cells were observed following treatment with the compounds for 48 hours. In silico studies, including molecular docking, molecular dynamics (MD) simulation, and MM/GBSA calculations, were carried out for the synthesized compounds against important cancer drug targets.ResultsThe highly cytotoxic agents QN10 and QN7 were identified with IC50 values of 4.49 +/- 0.68 mu M and 19.05 +/- 1.58 mu M in BT20 and HT29 cell lines, respectively. In addition, the morphologically changes were observed on MDA-MB-231 cells.ConclusionsThese findings show that the synthesized click products are highly cytotoxic agents in cancer cell lines and may be considered as potential candidates for enzyme inhibition.eninfo:eu-repo/semantics/closedAccessQuinoloneclick chemistryanticancermolecular dockingMD simulationArticle17131559157310.1080/17568919.2025.2533003Q2WOS:0015297913000012-s2.0-10501094211040667682Q2