Kavacik, MehmetInce, BaharAri, FikretUnal, Mehmet AltaySezgintürk, Mustafa KemalOzkan, Sibel A.2025-01-272025-01-2720230026-265X1095-9149https://doi.org/10.1016/j.microc.2023.109498https://hdl.handle.net/20.500.12428/20717Portable, fast, and practical devices are important in the fight against epidemic diseases. The lack of readily available small-scale commercial solutions prompted us to delve deeper into this research effort. Investment in Quartz Tuning Fork (QTF)-based biosensor research is significant due to its seamless integration with miniaturised and portable devices. Within the scope of this study, a new, cost-effective, and versatile frequency analyser designed for detecting diseases, compatible with screens of different sizes, has been introduced. The modified QTF sensor occured mass-sensitive SARS-CoV-2 nucleocapsid protein (SARS-NP) detection with remarkable simplicity, sensitivity, and selectivity. The developed QTF-based biosensor has an impressive linear detection range ranging from 5 to 200 ng/mL and an exceptionally low limit of detection (LOD) value of 1.72 ng/ mL. The sensitivity of the developed QTF-based biosensor was obtained as 50 Hz/1 mu M. The biosensor response was then evaluated in commercial human serum sample. After 15 days of storage, these biosensors retained approximately 93.7 % of their initial activity. Additionally, a Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM) comprehensively characterised the electrode surface.eninfo:eu-repo/semantics/closedAccessCOVID-19Quartz Tuning ForkQTFSARS-CoV-2BiosensorImmunosensorArticle19510.1016/j.microc.2023.109498Q1WOS:0011045799000012-s2.0-85174634104Q1