Akurut, CisemSılan, FatmaYalçıntepe, SinemÖzdemir, Öztürk2025-01-272025-01-2720192458-88652459-1505https://search.trdizin.gov.tr/tr/yayin/detay/372010https://hdl.handle.net/20.500.12428/20107Introduction: The non-invasive prenatal diagnosis of the fetus RhD genotype in RhD incompatibility has a crucial role in the prevention ofincreased anti-D immunoglobulin therapy for haemolytic diseases in pregnant women carrying RhD negative fetus. It was aimed to detect fetalRhD genotyping by using maternal circulating cell-free DNA in the current study.Methods: Maternal blood samples were collected in different trimester of pregnancies (12-40 weeks) in 12 D-negative mothers. Cell-free fetalDNA was extracted from 2 ml of maternal plasma by an conventional DNA isolation technique (Qiagen, Hilden, Germany) and real-time PCRwas performed for genotyping target RhD exons 7 and 10 and GLO genes. Postnatal serological evaluations were performed and the results wereconfirmed.Results: 6 cases (50 %) were determined D positive and 6 cases (50 %) were determined D negative. All results were also confirmed after birthserologically.Conclusions: In conclusion, the current results showed us the non-invasive target RhD genotyping from cell free fetal DNA from maternalplasma samples have a diagnostic accuracy in RhD incompatibility pregnancies.eninfo:eu-repo/semantics/openAccessKadın Hastalıkları ve DoğumHalk ve Çevre SağlığıArticle4116372010