Ozay, HavaTarimeri, NurGungor, ZeynepDemirbakan, BurcakOzcan, BurcuSezgintürk, Mustafa KemalÖzay, Özgür2025-01-272025-01-2720202365-6549https://doi.org/10.1002/slct.202002327https://hdl.handle.net/20.500.12428/26075In this study, for the first time in the literature, synthesis of Au(0) nanoparticles supported by a crosslinked gel structure was performed via enzyme-mediated reduction of Au(III) ions without using any chemical reductant. In our newly-developed method, glucose oxidase enzymes immobilized in the crosslinked gelatine structure ensured simultaneous reduction of the Au(III) ions diffused within the gel to Au(0). The Au@Gel obtained was structurally characterised with TEM (Transmission electron microscopy), EDX (Energy dispersive X-ray analysis) elemental mapping, XPS (X-Ray photoelectron spectroscopy) and XRD (X-Ray Diffraction) analyses. The catalytic activity of Au(0) particles with nearly 8 nm size in the Au@Gel was investigated for the reduction of 4-nitrophenol (4-NP) as a model compound in the presence of NaBH(4)as reducing agent. The activation parameters for the reduction reaction of 4-nitrophenol in the presence of Au@Gel catalyst were determined as E-a= 30.16 kJmol(-), Delta H= 27.52 kJmol(-)and Delta S= -197.45 Jmol(-)K(-). The Au@Gel catalyst system, with good catalytic activity, simultaneously has nearly perfect reusability.eninfo:eu-repo/semantics/closedAccessCatalytic reductionEnzymeGlucose OxidaseGoldHydrogel4-NitrophenolArticle5299143915210.1002/slct.202002327Q3WOS:0005576665000292-s2.0-85089185254Q3