Surgun-Acar, Yonca2025-01-272025-01-2720220269-74911873-6424https://doi.org/10.1016/j.envpol.2022.120262https://hdl.handle.net/20.500.12428/25297Micro and nanoplastics are new generation contaminants of global concern. It is important to evaluate the effects on edible products due to the presence of micro-and nano-sized plastics in the treated wastewater. A hydroponic experiment was carried out to explore the effect of polsytrene nanoplastics (PS-NPs; 20 nm) at different con-centrations (0, 12.5, 25, and 50 mg L-1) on Glycine max L. (soybean) seedlings for 7-days. In the current study, firstly the uptake of PS-NPs by Glycine max L. (soybean) roots were confirmed by laser confocal scanning mi-croscope. Exposure to PS-NPs, negatively affected growth parameters and increased Fe, Zn and Mn contents in roots and leaves of soybean seedlings. PS-NPs treatments caused oxidative stress in soybean seedlings. The hydrogen peroxide and malondialdehyde contents, showed similar increase pattern in seedlings exposed to PS -NPs. Response to PS-NPs, the level of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase) and proline content were generally enhanced in roots and leaves of soybean. The expression level of stress-related genes examined in the study included CSD5, FSD3, APX1, and POD up-regulated in PS-NPs treated-soybean seedlings in a tissue specific manner. The results of the present study showed the adverse effects of PS-NPs on soybean seedlings, which may have important implications for the risk assessment of NPs on crop production and environmental safety.eninfo:eu-repo/semantics/closedAccessGlycine maxNanoplasticsGrowthAntioxidative systemNutrientsGene expressionArticle31410.1016/j.envpol.2022.120262Q1WOS:0008705359000012-s2.0-8513936816736162560Q1