Cömert Önder, FerahSağbaş Suner, SelinŞahiner, NurettinAy, MehmetÖzpolat, Bülent2025-01-272025-01-2720200724-87411573-904Xhttps://doi.org/10.1007/s11095-020-2774-5https://hdl.handle.net/20.500.12428/22740Purpose To evalauted natural polymeric biomaterials including hyaluronic acid (HA) and its copolymeric form HA:Suc nanoparticles (NPs) as drug carrier systems for delivery of hydrophobic small molecule kinase EF2-kinase inhibitor in breast and pancreatic cancer cells. Methods In vitro cellular uptake studies of Rhodamine 6G labaled HA:Suc nanoparticles were evaluated by using flow cytometry analysis and fluorescent microscopy in breast (MDA-MB-231 and MDA-MB-436) and pancreatic cancer cells (PANC-1 and MiaPaca-2). Besides, in vitro release study of compound A (an EF2-kinase inhibitor) as a model hydrophobic drug was performed in the cancer cells. Results These biological evaluation studies indicated that HA and HA:Suc NPs provided a highly effective delivery of compound A were into breast and pancreatic cancer cells, leading to significant inhibition of cell proliferation and colony formation of breast and pancreatic cancer cells. Conclusion HA-sucrose NPs incorporating an EF2-Kinase inhibitor demonstrate significant biologic activity in breast and pancreatic cancer cells. This is the first study that shows natural polymeric drug carriers succesfully deliver a hydrofobic cancer drug into cancer cells. Nanoparticles based on HA:Suc are effective in delivering hydrofobic cancer drugs in breast and pancreatic cancers.eninfo:eu-repo/semantics/closedAccessbreast and pancreatic cancerelongation factor 2 kinasekinase inhibitorhyaluronic acid-sucrose nanoparticlestargeted therapyArticle37310.1007/s11095-020-2774-5Q2WOS:0005181849000012-s2.0-8508104126832133571Q1