Sahutoglu, A. SercanAkgul, Cahit2025-01-272025-01-2720150366-63521336-9075https://doi.org/10.1515/chempap-2015-0031https://hdl.handle.net/20.500.12428/21894Cross-linked enzyme aggregates (CLEA) of Aspergillus oryzea a-amylase (AoAA) and Aspergillus niger glucoamylase (AnGA) were prepared using glutaraldehyde and dextran polyaldehyde as crosslinkers. The maximum activity recoveries for glutaraldehyde cross-linking were 21.8 % and 41.2 %, respectively. The addition of a proteic feeder (bovine serum albumin) exhibited a negative effect on the activity recoveries for both enzymes. Dextran polyaldehyde was used as a cross-linking agent instead of glutaraldehyde to reduce the activity losses. As a result, an activity recovery of 60.0 % was obtained for Aspergillus oryzea a-amylase. On the other hand, no activity recovery was observed for Aspergillus niger glucoamylase due to the latter enzymes affinity for dextran.(C) 2014 Institute of Chemistry, Slovak Academy of Scienceseninfo:eu-repo/semantics/closedAccessimmobilisationglucoamylasealpha-amylaseglutaraldehydedextran polyaldehydecrosslinked enzyme aggregates (CLEAs)Article69343343910.1515/chempap-2015-0031Q3WOS:0003501349000072-s2.0-84922215083Q2