Teke, ZaferBostanci, Erdal BirolYenisey, CigdemKelten, Esra CananSacar, MustafaSimsek, Nilufer GencDuzcan, Suleyman Ender2025-01-272025-01-2720130894-19391521-0553https://doi.org/10.3109/08941939.2012.687434https://hdl.handle.net/20.500.12428/23980Purpose: We aimed to investigate whether caffeic acid phenethyl ester (CAPE) prevents detrimental systemic effects of intestinal ischemia-reperfusion (IR) injury on colonic anastomotic wound healing. Methods: This experimental study was conducted on 48 male Wistar albino rats. The rats were randomly allocated into four groups and a left colonic anastomosis was performed in all rats: (i) sham-operated group (n = 12), laparatomy without intestinal IR injury; (ii) sham + CAPE group (n = 12), identical to Group 1 except for CAPE treatment (10 mu mol/kg, intravenously); (iii) intestinal IR group (n = 12), 60 min of superior mesenteric ischemia followed by reperfusion; and (iv) IR + CAPE-treated group (n = 12) (10 mu mol/kg, intravenously, 30 min before the construction of colonic anastomosis). On the postoperative day 7, the rats were subjected to relaparotomy for in vivo measurement of the colonic anastomotic bursting pressure. A colonic segment including the anastomotic site was resected for histopathological evaluation and biochemical analyses. The plasma proinflammatory cytokine levels were measured. Body weight changes were examined. Results: CAPE treatment significantly increased colonic anastomotic bursting pressures, and colonic anastomotic tissue hydroxyproline contents and antioxidant parameters (p < .05), and significantly decreased oxidative stress markers in colonic anastomotic tissues and plasma proinflammatory cytokine levels (p < .05). Histopathological scores were significantly better due to CAPE administration (p < .05). Conclusions: This study clearly showed that CAPE treatment prevented the delaying effects of remote IR injury on colonic anastomotic wound healing. Further clinical studies are required to determine whether CAPE has a useful role in the enhancement of gastrointestinal anastomotic wound healing during particular surgeries in which IR-induced organ injury occurs.eninfo:eu-repo/semantics/closedAccesscaffeic acid phenethyl esterischemia-reperfusioncolonic anastomosiswound healingbursting pressureshydroxyprolinemyeloperoxidasemalondialdehydexanthine oxidasenitric oxideglutathioneglutathione peroxidaseglutathione reductasecatalaseArticle261162910.3109/08941939.2012.687434Q3WOS:0003146504000042-s2.0-8487367589522646141Q1