The First Identified Citrus tristeza virus Isolate of Turkey Contains a Mixture of Mild and Severe Strains

Basit Öğe Kaydı
dc.contributor.authorCevik, Bayram
dc.contributor.authorYardimci, Nejla
dc.contributor.authorKorkmaz, Savaş
dc.date.accessioned2025-01-27T20:26:54Z
dc.date.available2025-01-27T20:26:54Z
dc.date.issued2013
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe presence of Citrus tristeza virus (CTV) has previously been reported in citrus growing regions of Turkey. All serologically and biologically characterized isolates including Igdir, which was the first identified CTV isolates from Turkey, were considered mild isolates. In this study, molecular characteristics of the Igdir isolate were determined by different methods. Analysis of the Igdir isolate by western blot and BD-RT-PCR assays showed the presence of MCA13 epitope, predominantly found in severe isolates, in the Igdir isolate revealing that it contains a severe component. For further characterization, the coat protein (CP) and the RNA-dependent RNA polymerase (RdRp) genes representing the 3' and 5' half of CTV genome, respectively, were amplified from dsRNA by RT-PCR. Both genes were cloned separately and two clones for each gene were sequenced. Comparisons of nucleotide and deduced amino acid sequences showed that while two CP gene sequences were identical, two RdRp clones showed only 90% and 91% sequence identity in their nucleotide and amino acid sequences, respectively, suggesting a mixed infection with different strains. Phylogenetic analyses of the CP and RdRp genes of Igdir isolate with previously characterized CTV isolates from different citrus growing regions showed that the CP gene was clustered with NZRB-TH30, a resistance breaking isolate from New Zealand, clearly showing the presence of severe component. Furthermore, two different clones of the RdRp gene were clustered separately with different CTV isolates with a diverse biological activity. While the RdRp-1 was clustered with T30 and T385, two well-characterized mild isolates from Florida and Spain, respectively, the RdRp-2 was most closely related to NZRB-G90 and NZRB-TH30, two well-characterized resistance breaking and stem pitting (SP) isolates from New Zealand confirming the mixed infection. These results clearly demonstrated that the Igdir isolate, which was previously described as biologically a mild isolate, actually contains a mixture of mild and severe strains.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [104O584]
dc.description.sponsorshipThis study was conducted by a grant from the Scientific and Technological Research Council of Turkey (TUBITAK) project number 104O584.
dc.identifier.doi10.5423/PPJ.OA.09.2012.0141
dc.identifier.endpage41
dc.identifier.issn1598-2254
dc.identifier.issn2093-9280
dc.identifier.issue1
dc.identifier.pmid25288926
dc.identifier.scopus2-s2.0-84884373388
dc.identifier.scopusqualityQ1
dc.identifier.startpage31
dc.identifier.urihttps://doi.org/10.5423/PPJ.OA.09.2012.0141
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22494
dc.identifier.volume29
dc.identifier.wosWOS:000315612200004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherKorean Soc Plant Pathology
dc.relation.ispartofPlant Pathology Journal
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectCitrus tristeza virus
dc.subjectCoat protein
dc.subjectPhylogenetic analysis
dc.subjectRNA-dependent RNA polymerase
dc.subjectStrain identification
dc.titleThe First Identified Citrus tristeza virus Isolate of Turkey Contains a Mixture of Mild and Severe Strains
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu