Quartz crystal microbalance-based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples

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dc.authoridBeyazıt, Fatma / 0000-0002-0667-6090
dc.contributor.authorBeyazıt, Fatma
dc.contributor.authorArıca, Mehmet Yakup
dc.contributor.authorAçıkgöz-Erkaya, İlkay
dc.contributor.authorÖzalp, Veli Cengiz
dc.contributor.authorBayramoğlu, Gülay
dc.date.accessioned2025-01-27T20:59:41Z
dc.date.available2025-01-27T20:59:41Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA fast and accurate identification of Listeria monocytogenes. A new quartz crystal microbalance (QCM) aptasensor was designed for the specific and rapid detection of L. monocytogenes. Before detection of the target bacterium from samples in the QCM aptasensor, a magnetic pre-enrichment system was used to eliminate any contaminant in the samples. The prepared magnetic system was characterized using ATR-FTIR, SEM, VSM, BET, and analytical methods. The saturation magnetization values of the Fe3O4, Fe3O4@PDA, and Fe3O4@PDA@DAPEG particles were 57.2, 40.8, and 36.4 emu/g, respectively. The same aptamer was also immobilized on the QCM crystal integrated into QCM flow cell and utilized to quantitatively detect L. monocytogenes cells from the samples. It was found that a specific aptamer-magnetic pre-concentration system efficiently captured L. monocytogenes cells in a short time (approximately 10 min). The Fe3O4@PDA@DA-PEG-Apt particles provided selective isolation of L. monocytogenes from the bacteria-spiked media up to 91.8%. The immobilized aptamer content of the magnetic particles was 5834 mu g/g using 500 ng Apt/mL. The QCM aptasensor showed a very high range of analytical performance to the target bacterium from 1.0 x 10(2) and 1.0 x 10(7) CFU/mL. The limit of detection (LOD) and limit of quantitation (LOQ) were 148 and 448 CFU/mL, respectively, from the feeding of the QCM aptasensor flow cell with the eluent of the magnetic pre-concentration system. The reproducibility of the aptasensor was more than 95%. The aptasensor was very specific to L. monocytogenes compared to the other Listeria species (i.e., L. ivanovii, L. innocua, and L. seeligeri) or other tested bacteria such as Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. The QCM aptasensor was regenerated with NaOH solution, and the system was reused many times.
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK) [119Z886]
dc.description.sponsorshipGB and VCO wish to acknowledge the support of the Scientific and Technological Research Council of Turkiye (TUBITAK) for this work (Project No: 119Z886). All the other authors acknowledge their respective universities and departments for their support during the preparation of this manuscript.
dc.identifier.doi10.1007/s00604-024-06307-2
dc.identifier.issn0026-3672
dc.identifier.issn1436-5073
dc.identifier.issue5
dc.identifier.pmid38570380
dc.identifier.scopus2-s2.0-85189440574
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s00604-024-06307-2
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26787
dc.identifier.volume191
dc.identifier.wosWOS:001197236200006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Wien
dc.relation.ispartofMicrochimica Acta
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectMagnetic particles
dc.subjectPre-concentration
dc.subjectAptasensor
dc.subjectQuartz crystal microbalance
dc.subjectListeria monocytogenes detection
dc.titleQuartz crystal microbalance-based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples
dc.typeArticle

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