A new method for the detection of minor populations of citrus tristeza virus strains infecting single citrus trees

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dc.contributor.authorGen-cec, Hanife
dc.date.accessioned2025-01-27T19:00:26Z
dc.date.available2025-01-27T19:00:26Z
dc.date.issued2005
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractFifteen different biologically important citrus tristeza virus (CTV) isolates (B14, B15, B23, B31, B52, B57, T36, D9, D10, D11, D12, D15, D16, D20 and D32)were tested using strain-specific biotin-labeled oligonucleoticle probes. Coat protein genes (CPGs) of the samples were amplified by PCR, and the products were hybridized with probes (direct PCR hybridization (DPH)) and sequenced. In the DPH, all samples having probe O reactions were infected with CTV. Some isolates giving more than one specific probe reaction showed that they may be infected by mixtures of different CTV strains. In order to investigate this, CPGs from individual bacterial colonies were used as PCR templates and the PCR products were tested. This method developed in this study was called colony PCR hybridization (CPH). It was found that samples such as B57, which were apparently mixtures of strains, were indeed mixtures and other samples, which apparently contained a single strain (B23, D9, D11 and D16), actually contained one or more additional strains not detectable by DPH or other methods. CPH is a sensitive method for the detection and differentation of CTV strains infecting single citrus trees. © TÜBİTAK.
dc.identifier.doi10.3906/tar-0410-5
dc.identifier.endpage459
dc.identifier.issn1300-011X
dc.identifier.issue6
dc.identifier.scopus2-s2.0-28844476714
dc.identifier.scopusqualityQ1
dc.identifier.startpage449
dc.identifier.trdizinid48683
dc.identifier.urihttps://doi.org/10.3906/tar-0410-5
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/48683
dc.identifier.urihttps://hdl.handle.net/20.500.12428/13320
dc.identifier.volume29
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTurkiye Klinikleri Journal of Medical Sciences
dc.relation.ispartofTurkish Journal of Agriculture and Forestry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_Scopus_20250125
dc.subjectColony PCR hybridization; CTV; Hybridization; PCR; Probes
dc.titleA new method for the detection of minor populations of citrus tristeza virus strains infecting single citrus trees
dc.typeArticle

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