A novel silanization agent based single used biosensing system: Detection of C-reactive protein as a potential Alzheimer's disease blood biomarker

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dc.authoridSonuc Karaboga, Munteha Nur/0000-0002-3153-1328
dc.contributor.authorKaraboga, Munteha Nur Sonuc
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:41:16Z
dc.date.available2025-01-27T20:41:16Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThis paper illustrates a new and sensitive electrochemical immunosensor for the analysis of C-reactive protein. Indium Tin Oxide (ITO) disposable sheets were modified by using 3-cyanopropyltrimethoxysilane (CPTMS) self-assembled monolayers (SAMs) for the first time for immobilizing the anti-CRP antibody via covalent interactions without the need for any cross-linking agent. Cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), as well as square wave voltammetry (SWV) methods were applied to characterize immobilization steps of anti-CRP and to determine the CRP concentration. The optimization of the fabricated parameters and the analytical performance of the biosensor were widely evaluated. Charge transfer resistance changes were highly linear and sensitive with CRP concentration of 3.25-208 fg mL(-1) range and associated with a limit of detection of 0.455 fg mL(-1). This impedimetric biosensing system have excellent repeatability, reproducibility and reusability. Moreover, the binding characterization of CRP to anti-CRP was monitored by a single frequency impedance technique. The amount of CRP in human serum samples were analyzed by fabricated biosensor to determine the feasibility of the biosensing system in medical purposes. We suggest that CPTMS, a new silanization agent, is ideal in biosensor applications. (C) 2018 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [113 Z 678]
dc.description.sponsorshipThis work was funded by TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) whose assistance is greatly acknowledged.
dc.identifier.doi10.1016/j.jpba.2018.03.016
dc.identifier.endpage235
dc.identifier.issn0731-7085
dc.identifier.issn1873-264X
dc.identifier.pmid29558723
dc.identifier.scopus2-s2.0-85043994209
dc.identifier.scopusqualityQ1
dc.identifier.startpage227
dc.identifier.urihttps://doi.org/10.1016/j.jpba.2018.03.016
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24083
dc.identifier.volume154
dc.identifier.wosWOS:000431285100027
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysis
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subject3-cyanopropyltrimethoxysilane
dc.subjectC-reactive protein (CRP)
dc.subjectBiosensor
dc.subjectElectrochemical impedance spectroscopy (EIS)
dc.subjectAlzheimer's disease
dc.titleA novel silanization agent based single used biosensing system: Detection of C-reactive protein as a potential Alzheimer's disease blood biomarker
dc.typeArticle

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